John Dallon: Paper Abstract

Modeling the Effects of TGFbeta on Extracellular Matrix Alignment in Dermal Wound Repair


AUTHORS:

John C. Dallon 1, Jonathan. A. Sherratt 2 and Philip K. Maini 3

1: Department of Mathematics, Brigham Young University, Provo, UT 84602

2: Department of Mathematics, Heriot-Watt University, Edinburgh EH14 4AS, UK

3: Centre for Mathematical Biology, Mathematical Institute, University of Oxford, 24-29 St Giles', Oxford OX1 3LB, UK


ABSTRACT:

We present a novel mathematical model for collagen deposition and alignment during dermal wound healing, focusing on the regulatory effects of TGFbeta.. Our work extends a previously developed model which considers the interactions between fibroblasts and extracellular matrix, composed of collagen and a fibrin based blood clot, by allowing fibroblasts to orient the collagen matrix, and produce and degrade the extracellular matrix, while the matrix can direct the fibroblasts and control their speed. Here we extend the model by allowing a time varying concentration of TGFbeta to alter the properties of the fibroblasts. Thus we are able to simulate experiments which alter the TGFbeta profile. Within this model framework we find that most of the known effects of TGFbeta, i.e., changes in cell motility, cell proliferation and collagen production, are of minor importance to matrix alignment and cannot explain the anti-scarring properties of TGFbeta. However, we find that by changing fibroblast reorientation rates, consistent with experimental evidence, the alignment of the regenerated tissue can be significantly altered. This provides an explanation for the experimentally observed influence of TGFbeta on scarring.


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